4E8R
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of Cs+ and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4861 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.200, 94.915, 225.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.500 - 3.360 |
| R-factor | 0.20325 |
| Rwork | 0.200 |
| R-free | 0.25799 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3igi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.392 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.503 | 3.450 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rmerge | 0.081 | 0.967 |
| Number of reflections | 26102 | |
| <I/σ(I)> | 15.1 | 2 |
| Completeness [%] | 97.5 | 96.8 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM magnesium acetate, 200 mM cesium chloride, 50 mM HEPES sodium, pH 7.0, 4% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
