4E68
Unphosphorylated STAT3B core protein binding to dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91730 |
| Spacegroup name | I 41 |
| Unit cell lengths | 174.140, 174.140, 79.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.585 |
| R-factor | 0.22778 |
| Rwork | 0.225 |
| R-free | 0.27332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.732 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.160 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.056 | 0.527 |
| Number of reflections | 36458 | |
| <I/σ(I)> | 15.2 | 2.3 |
| Completeness [%] | 97.6 | 99.3 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.005M MgSO4, 0.1M ammonium acetate, 10% glycerol, 0.05M MES pH 5.6, 0.25M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
