4E3Y

X-ray structure of the Serratia marcescens endonuclease at 0.95 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-10-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.847
Spacegroup name	P 21 21 2
Unit cell lengths	106.265, 73.418, 67.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 0.950
R-factor	0.1309
Rwork	0.130
R-free	0.14530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g8t
RMSD bond length	0.017
RMSD bond angle	1.820
Data reduction software	DENZO
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.597
High resolution limit [Å]	0.950
Number of reflections	313007
Completeness [%]	94.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	298	10 mM TRIS-HCl, 1 mM Mg(2+), PEG4000, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K