4E2F
Crystal Structure of E. coli Aspartate Transcarbamoylase K164E/E239K Mutant in an intermediate state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 124.086, 144.814, 203.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.888 - 2.800 |
R-factor | 0.2171 |
Rwork | 0.214 |
R-free | 0.27420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.511 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.890 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.081 | 0.473 |
Number of reflections | 83815 | |
<I/σ(I)> | 8.7 | 2.2 |
Completeness [%] | 92.4 | 92.4 |
Redundancy | 3.33 | 3.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.75 | 293 | 16% (w/v) PEG 4000, 0.04 M Na2MoO4, 0.04 M N-cyclohexyl-3-aminopropanesulfonic acid, 30 mM Tris-acetate, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |