4E0X
Crystal structure of the kainate receptor GluK1 ligand-binding domain in complex with kainate in the absence of glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.256, 68.256, 235.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.513 - 2.000 |
R-factor | 0.1769 |
Rwork | 0.175 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c32 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.004 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.513 | 29.513 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.025 | 0.411 | |
Total number of observations | 7832 | 35466 | |
Number of reflections | 38773 | ||
<I/σ(I)> | 9.2 | 20.9 | 1.8 |
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 6.4 | 5.6 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 280 | 20% PEG4000, 0.35M lithium sulfate, 0.1M sodium cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |