4DXD
Staphylococcal Aureus FtsZ in complex with 723
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.715, 54.650, 84.496 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 40.460 - 2.010 |
| R-factor | 0.1892 |
| Rwork | 0.188 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.9.7) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.460 |
| High resolution limit [Å] | 2.010 |
| Number of reflections | 19629 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1M ammonium sulfate, 0.1M TRIS pH8.5 and 25% PEG3350, VAPOR DIFFUSION, temperature 293K |
