4DXA

Co-crystal structure of Rap1 in complex with KRIT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	57.548, 77.970, 59.757
Unit cell angles	90.00, 90.93, 90.00

Refinement procedure

Resolution	47.425 - 1.950
R-factor	0.1983
Rwork	0.196
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c1y
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	37653
<I/σ(I)>	15.4	2.56
Completeness [%]	97.2	98.1
Redundancy	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2M KNO3, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K