4DW3
Crystal structure of the glycoprotein Erns from the pestivirus BVDV-1 in complex with 5'-CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2008-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.930, 106.460, 64.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.330 - 2.350 |
R-factor | 0.1879 |
Rwork | 0.186 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dvk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.930 | 2.360 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 19939 | |
Completeness [%] | 96.0 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 298 | 33% PEG2000 MME, 100mM Na-Acetate, 140mM (NH4)2SO4, 50mM KH2PO4, pH 4.6, vapor diffusion, temperature 298K |