4DQO
Crystal Structure of PG16 Fab in Complex with V1V2 Region from HIV-1 strain ZM109
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Collection date | 2011-01-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.054, 207.561, 87.617 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.112 - 2.438 |
R-factor | 0.2028 |
Rwork | 0.201 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mug |
RMSD bond length | 0.002 |
RMSD bond angle | 0.723 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.174 | |
Number of reflections | 25408 | |
<I/σ(I)> | 8.7 | 1.4 |
Completeness [%] | 91.0 | 61.4 |
Redundancy | 5.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% Isopropanol, 15% PEG 3550, 0.2M ammonium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |