4DQO
Crystal Structure of PG16 Fab in Complex with V1V2 Region from HIV-1 strain ZM109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.054, 207.561, 87.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.112 - 2.438 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mug |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.723 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.174 | |
| Number of reflections | 25408 | |
| <I/σ(I)> | 8.7 | 1.4 |
| Completeness [%] | 91.0 | 61.4 |
| Redundancy | 5.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% Isopropanol, 15% PEG 3550, 0.2M ammonium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
