4DQ2
Structure of staphylococcus aureus biotin protein ligase in complex with biotinol-5'-amp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 298 |
| Collection date | 2009-09-09 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 94.598, 94.598, 130.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.550 - 2.500 |
| R-factor | 0.19341 |
| Rwork | 0.191 |
| R-free | 0.23932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.214 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.550 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 20793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10% PEG 8000, 0.1M Tris-HCl pH 7.5, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
