4DPG
Crystal Structure of Human LysRS: P38/AIMP2 Complex I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.200, 122.010, 149.200 |
| Unit cell angles | 89.16, 85.58, 89.71 |
Refinement procedure
| Resolution | 46.836 - 2.844 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.194 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.960 |
| High resolution limit [Å] | 2.844 | 6.160 | 2.844 |
| Rmerge | 0.102 | 0.036 | 0.487 |
| Number of reflections | 128809 | ||
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 99.1 | 99.7 | 98.6 |
| Redundancy | 4 | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 291 | 22% PEG10000, 0.1 M MES pH 6.7, 0.1 M magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
