4DOU
Crystal Structure of a Single-chain Trimer of Human Adiponectin Globular Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.310, 49.630, 50.460 |
| Unit cell angles | 117.23, 101.68, 105.31 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2029 |
| Rwork | 0.200 |
| R-free | 0.25693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c28 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.323 |
| Number of reflections | 72800 | |
| <I/σ(I)> | 10.5 | 3 |
| Completeness [%] | 91.0 | 72.2 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 22% PEG3350, 0.1M Bis-Tris, and 25% PEG200, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
