4DLQ
Crystal structure of the GAIN and HormR domains of CIRL 1/Latrophilin 1 (CL1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 65 |
Detector technology | CCD |
Collection date | 2009-07-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 124.000, 124.000, 77.305 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.900 - 1.850 |
R-factor | 0.1646 |
Rwork | 0.164 |
R-free | 0.18540 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.092 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.900 | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
Number of reflections | 58546 | ||
Completeness [%] | 99.7 | 99.8 | 95.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.26M (NH4)2SO4, 0.1M sodium acetate pH 4.5, 0.2M NaCl,, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.26M (NH4)2SO4, 0.1M sodium acetate pH 4.5, 0.2M NaCl,, VAPOR DIFFUSION, HANGING DROP, temperature 293K |