4DLA
Crystal structure of S-nitrosoglutathione reductase apoenzyme from tomato (Solanum lycopersicum)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-14 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.710, 125.000, 77.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.250 - 2.140 |
| R-factor | 0.2401 |
| Rwork | 0.237 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1teh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.250 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.111 | 0.578 |
| Number of reflections | 43940 | |
| <I/σ(I)> | 9.21 | 2.59 |
| Completeness [%] | 97.6 | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 0.1 M MES pH 6.5, 12% PEG 20000, 4% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
