4DKX
Crystal Structure of the Rab 6A'(Q72L)
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-11-09 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.999 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 36.838, 96.780, 109.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.390 - 1.900 |
R-factor | 0.19913 |
Rwork | 0.196 |
R-free | 0.25036 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 3.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.390 | |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 31899 | |
Completeness [%] | 70.0 | 70 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG 8000, 200mM Calcium acetate, 100mM Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |