4DGY

Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody HCV1, C2 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	141.099, 63.065, 69.300
Unit cell angles	90.00, 95.72, 90.00

Refinement procedure

Resolution	28.764 - 1.798
R-factor	0.1893
Rwork	0.188
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3giz
RMSD bond length	0.007
RMSD bond angle	1.074
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.198	1.860
High resolution limit [Å]	1.798	1.798
Number of reflections	55279
<I/σ(I)>	13.4	3
Completeness [%]	98.9	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	25% PEG4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K