4DE4
Crystal structure of aminoglycoside phosphotransferase APH(2")-Id/APH(2")-IVa in complex with HEPES
Replaces: 3R80Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.541, 101.214, 71.166 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 28.988 - 2.000 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.27220 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39540 | |
| <I/σ(I)> | 35.71 | 3.11 |
| Completeness [%] | 97.9 | 97.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M NASCN, 20% PEG3350, 2 MM GTP, 2.5 MM GENTAMICIN, PH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
