4DDI
Crystal structure of human OTUB1/UbcH5b~Ub/Ub
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.739, 106.225, 134.689 |
| Unit cell angles | 90.00, 123.74, 90.00 |
Refinement procedure
| Resolution | 49.469 - 3.802 |
| R-factor | 0.2212 |
| Rwork | 0.218 |
| R-free | 0.27310 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 4.020 |
| High resolution limit [Å] | 3.796 | 3.796 |
| Number of reflections | 19737 | |
| Completeness [%] | 97.0 | 83.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 25% PEG1500, 0.1 M SPG, pH 8.0, VAPOR DIFFUSION, temperature 293K |
