4DCH
Insights into Glucokinase Activation Mechanism: Observation of Multiple Distinct Protein Conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-07-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.917, 85.794, 72.939 |
| Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.790 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.472 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.068 | 0.052 | 0.408 |
| Number of reflections | 54275 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 97.2 | 99 | 75.5 |
| Redundancy | 3.4 | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 286 | 20% PEG 3350, 0.1A citrate pH 5.4, 0.2M ammonium iodide, 50mM glucose, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
