4D9O

Structure of ebolavirus protein VP24 from Reston

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	38.438, 103.890, 59.773
Unit cell angles	90.00, 93.94, 90.00

Refinement procedure

Resolution	31.300 - 2.000
R-factor	0.189
Rwork	0.186
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000	2.000
Number of reflections	31125
<I/σ(I)>	21
Completeness [%]	98.7	98.7
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	295	0.1M Bis-Tris, 0.2M NaCl, 8% PEG3350, 14%(w/v) D-(+)-sucrose, pH 5.5, VAPOR DIFFUSION, temperature 295K