4D1U
A D120A mutant of VIM-7 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 43 |
Unit cell lengths | 69.764, 69.764, 47.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.665 - 1.800 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.20750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y8b |
RMSD bond length | 0.012 |
RMSD bond angle | 1.298 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.540 |
Number of reflections | 21271 | |
<I/σ(I)> | 13.2 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |