4D11
GalNAc-T2 crystal soaked with UDP-5SGalNAc, mEA2 peptide and manganese (Lower resolution dataset)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 116.771, 120.898, 249.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 249.610 - 2.850 |
| R-factor | 0.21655 |
| Rwork | 0.216 |
| R-free | 0.23497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ffv |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.927 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 83233 | |
| <I/σ(I)> | 13.5 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
