4D05
Structure and activity of a minimal-type ATP-dependent DNA ligase from a psychrotolerant bacterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.427, 43.976, 89.633 |
| Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
| Resolution | 24.610 - 1.650 |
| R-factor | 0.1468 |
| Rwork | 0.145 |
| R-free | 0.19240 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.439 |
| Data reduction software | XDS |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 80785 | |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.75 M AMMONIUM SULFATE, 100 MM HEPES PH 7.4 |
