4CZM

C. crescentus MreB, monomeric, AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS
Spacegroup name	P 43 21 2
Unit cell lengths	67.791, 67.791, 320.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.138 - 2.200
R-factor	0.212
Rwork	0.209
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jce
RMSD bond length	0.018
RMSD bond angle	1.893
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	1.500
Number of reflections	39419
<I/σ(I)>	19.5	1.8
Completeness [%]	100.0	100
Redundancy	12.9	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1