4CZF
C. crescentus MreB, single filament, ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.548, 71.815, 53.731 |
| Unit cell angles | 90.00, 100.37, 90.00 |
Refinement procedure
| Resolution | 21.406 - 1.640 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jce |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.630 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.030 | 0.150 |
| Number of reflections | 46866 | |
| <I/σ(I)> | 42.1 | 10.7 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
