4CUE
Human Notch1 EGF domains 11-13 mutant T466V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.780, 63.780, 127.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.670 - 3.000 |
| R-factor | 0.2608 |
| Rwork | 0.260 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.670 | 3.350 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 3174 | |
| <I/σ(I)> | 10.2 | 2.8 |
| Completeness [%] | 94.1 | 94.3 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
