4CUE

Human Notch1 EGF domains 11-13 mutant T466V

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1	polymer	135	14855.6	1	UniProt (P46531) Pfam (PF07645) Pfam (PF00008) In PDB	HOMO SAPIENS (HUMAN)	NOTCH 1, HN1, TRANSLOCATION-ASSOCIATED NOTCH PROTEIN TAN-1, HUMAN NOTCH
2	A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
3		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 411 - 526 (UniProt: P46531)

PDB	External Database	Details
Ser 409	-	expression tag
Ala 410	-	expression tag
Val 527	-	expression tag
Asp 528	-	expression tag
Leu 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
Ile 532	-	expression tag
Leu 533	-	expression tag
Asp 534	-	expression tag
Ala 535	-	expression tag
Gln 536	-	expression tag
Lys 537	-	expression tag
Met 538	-	expression tag
Val 539	-	expression tag
Trp 540	-	expression tag
Asn 541	-	expression tag
His 542	-	expression tag
Arg 543	-	expression tag
Val 466	Thr 466	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	14855.6
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	14975.9

*Water molecules are not included.