4CT0
Crystal Structure of Mouse Cryptochrome1 in Complex with Period2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-03 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 99.736, 99.736, 178.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.496 - 2.450 |
R-factor | 0.1884 |
Rwork | 0.186 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k0r |
RMSD bond length | 0.009 |
RMSD bond angle | 1.125 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 45.500 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.060 | 1.050 |
Number of reflections | 33988 | |
<I/σ(I)> | 35 | 3.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.1 | 26.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M MGCL2, 12-13% PEG3350 |