4CS2
Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.179, 43.492, 63.266 |
| Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
| Resolution | 39.924 - 1.900 |
| R-factor | 0.1796 |
| Rwork | 0.178 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q7e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.920 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.490 |
| Number of reflections | 21692 | |
| <I/σ(I)> | 18.75 | 2.5 |
| Completeness [%] | 99.3 | 98.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 50MM TRIS-HCL PH 8.5, 200 MM CALCIUM CHLORIDE AND 18 % (W/V) PEG4000 |
