4CS0
Direct visualisation of strain-induced protein post-translational modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2013-12-03 |
Detector | RIGAKU R-AXIS IV |
Spacegroup name | I 4 |
Unit cell lengths | 86.270, 86.270, 80.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.690 - 2.100 |
R-factor | 0.17523 |
Rwork | 0.172 |
R-free | 0.23688 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4azd |
RMSD bond length | 0.019 |
RMSD bond angle | 2.094 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.180 | 0.620 |
Number of reflections | 17250 | |
<I/σ(I)> | 5.7 | 1.8 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 20% (W/V) POLYETHYLENE GLYCOL (PEG) 3350, 0.1 M BIS-TRIS PROPANE PH 7.4, 0.2 M POTASSIUM THIOCYANATE |