4CP8
Structure of the amidase domain of allophanate hydrolase from Pseudomonas sp strain ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-25 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.447, 179.232, 112.610 |
| Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.22577 |
| Rwork | 0.224 |
| R-free | 0.25883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dqn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 179.200 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.150 | 0.810 |
| Number of reflections | 107970 | |
| <I/σ(I)> | 12.6 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | PROTEIN AT 5.5 MG/ML WAS ADDED TO THE RESERVOIR CONDITION OF 11-14% (W/V) PEG 3350, 2% TACSIMATE PH 5. MICROSEEDS WERE ALSO ADDED TO THESE DROPS FOR REPRODUCIBLE CRYSTALS. |
