4COK
Functional and Structural Characterization of Pyruvate decarboxylase from Gluconoacetobacter diazotrophicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.070, 140.960, 91.060 |
| Unit cell angles | 90.00, 125.78, 90.00 |
Refinement procedure
| Resolution | 84.060 - 1.690 |
| R-factor | 0.13346 |
| Rwork | 0.132 |
| R-free | 0.16294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vbi |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.499 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.080 | 0.280 |
| Number of reflections | 146324 | |
| <I/σ(I)> | 6.5 | 2.7 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | BATCH CRYSTALLIZATION FROM 200 MM SODIUM CITRATE PH 6.0, 1 MM THDP, 1 MM MGCL2. PROTEIN CONCENTRATION WAS 4 MG/ML AND CRYSTALS APPEARED AFTER 4 DAYS. |
