4CNC
Crystal structure of human 5T4 (Wnt-activated inhibitory factor 1, Trophoblast glycoprotein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.600, 95.820, 65.970 |
| Unit cell angles | 90.00, 91.14, 90.00 |
Refinement procedure
| Resolution | 54.330 - 1.770 |
| R-factor | 0.17929 |
| Rwork | 0.177 |
| R-free | 0.21727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5T4 CRYSTAL FORM WITH 1 MOLECULE IN THE ASYMMETRIC UNIT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.330 | 1.820 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.060 | 0.760 |
| Number of reflections | 59830 | |
| <I/σ(I)> | 13.8 | 2.5 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | THE CRYSTALLIZATION SITTING DROP CONTAINED 100 NL OF CONCENTRATED 5T4/WAIF1 (5 MG/ML IN 20 MM TRIS-HCL, PH 7.5, 150 MM NACL) PLUS 100 NL OF 0.2 M (NH4)2SO4, 30 % W/V PEG 4000. |
