4CN5
Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human Nr1d1 Response Element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-08 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.286, 44.327, 63.915 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 43.093 - 2.000 |
| R-factor | 0.1705 |
| Rwork | 0.167 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.030 | 0.120 |
| Number of reflections | 19176 | |
| <I/σ(I)> | 27.2 | 9.4 |
| Completeness [%] | 98.3 | 98.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 20% PEG 3350, 0.2M KNO3, pH 6.6 |
