4CN2
Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human Ramp2 Response Element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-08 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.234, 43.952, 63.316 |
| Unit cell angles | 90.00, 106.02, 90.00 |
Refinement procedure
| Resolution | 23.898 - 2.069 |
| R-factor | 0.1674 |
| Rwork | 0.165 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.164 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 18563 | |
| <I/σ(I)> | 15.9 | 4.2 |
| Completeness [%] | 95.3 | 70.8 |
| Redundancy | 3.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG 3350, 0.2M NH4CL, 0.1M MGCL2, 0.1M MES PH 6 |
