4CMN
Crystal structure of OCRL in complex with a phosphate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 146.766, 146.766, 146.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.922 - 3.130 |
| R-factor | 0.2151 |
| Rwork | 0.210 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mtc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.780 | 3.300 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.060 | 0.790 |
| Number of reflections | 10068 | |
| <I/σ(I)> | 10.5 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40.5 | 42.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M ZINC ACETATE, 0.1 M NA-CACODYLATE PH 6.5, 10% ISOPROPANOL |
