4CLS
Crystal structure of human soluble Adenylyl Cyclase with Pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 |
| Unit cell lengths | 100.090, 100.090, 97.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.680 - 1.850 |
| R-factor | 0.17558 |
| Rwork | 0.174 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4clf |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.923 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.680 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.104 | 0.887 |
| Number of reflections | 47385 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M SODIUM ACETATE PH 4.8, 0.2 M TRI-SODIUM-CITRATE, 15% (W/V) PEG 4000, 10% (V/V) GLYCEROL |
