4CL7

Crystal structure of VEGFR-1 domain 2 in presence of Cobalt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-06
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	27.746, 41.711, 94.694
Unit cell angles	87.05, 82.25, 73.03

Refinement procedure

Resolution	19.469 - 2.000
R-factor	0.1707
Rwork	0.168
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1flt
RMSD bond length	0.006
RMSD bond angle	0.983
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.470	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	0.330
Number of reflections	26362
<I/σ(I)>	10.74	3.01
Completeness [%]	97.1	96.6
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		CRYSTAL WERE OBTAINED BY HANGING DROP METHOD FROM 1MM VEGFR1-D2, 5MM HEPES/NAOH PH7.0, 10MM COCL2, 100 MM BIS-TRIS/HCL PH6.5 15%(W/V) PEG3350.