Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.308, 59.308, 178.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.200 |
| R-factor | 0.20056 |
| Rwork | 0.198 |
| R-free | 0.24702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zos |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.960 |
| Number of reflections | 17062 | |
| <I/σ(I)> | 17.5 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 21% PEG3350, 0.1M BIS-TRIS-PROPANE PH 7.2, 0.2M SODIUM/POTASSIUM PHOSPHATE |
