4CKJ
Crystal structure of RET tyrosine kinase domain bound to adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.190, 69.090, 78.640 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 49.736 - 1.650 |
| R-factor | 0.1547 |
| Rwork | 0.153 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ivt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.838 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.670 |
| Number of reflections | 46229 | |
| <I/σ(I)> | 4.8 | 1.2 |
| Completeness [%] | 90.8 | 91 |
| Redundancy | 1.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
