4CIJ
Structure of Rolling Circle Replication Initiator Protein from Geobacillus stearothermophilus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.111, 136.848, 149.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.250 - 2.300 |
| R-factor | 0.19503 |
| Rwork | 0.193 |
| R-free | 0.23457 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.860 |
| Number of reflections | 60795 | |
| <I/σ(I)> | 16.9 | 2.2 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M PROLINE, 0.1 M HEPES PH 7.5, 10 % (V/V) PEG 3350 |
