4CID
Structural insights into the N-terminus of the EHD2 ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.851, 134.653, 56.068 |
| Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
| Resolution | 78.150 - 3.000 |
| R-factor | 0.23255 |
| Rwork | 0.230 |
| R-free | 0.27917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qpt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 26426 | |
| <I/σ(I)> | 10.5 | 1.8 |
| Completeness [%] | 95.9 | 98 |
| Redundancy | 1.63 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES PH 6.5, 20 MM MGCL2, 4% PEG3350, 5% MPD |
