4CI8
Crystal structure of the tandem atypical beta-propeller domain of EML1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.980, 83.980, 115.520 |
| Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
| Resolution | 47.375 - 2.600 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.25080 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.885 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 53683 | |
| <I/σ(I)> | 10.1 | 2.7 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100 MM TRI-SODIUM CITRATE PH 5.6, 2.0 M AMMONIUM SULPHATE, 200 MM POTASSIUM/SODIUM TARTRATE, 2 MM DTT. |
