4CGZ
Crystal structure of the Bloom's syndrome helicase BLM in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 42 |
| Unit cell lengths | 149.530, 149.530, 64.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.646 - 3.200 |
| R-factor | 0.2305 |
| Rwork | 0.229 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cdg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.133 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.040 | 1.500 |
| Number of reflections | 23593 | |
| <I/σ(I)> | 11.9 | 0.6 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 21% PEG 4000, 0.1M HEPES PH 7.6, 5% 2-PROPANOL |
