4CGY
Crystal structure of the human topoisomerase III alpha-RMI1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 10 |
| Detector technology | PIXEL |
| Collection date | 2010-09-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 94.010, 94.010, 381.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.490 - 2.850 |
| R-factor | 0.1989 |
| Rwork | 0.197 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1ECL AND 3NBI |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XDS (AIMLESS) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.780 | 2.970 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.150 | |
| Number of reflections | 41328 | |
| <I/σ(I)> | 16.7 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 100 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 8-12% (W/V) PEG 2000, 100 MM TRIS-HCL PH 7.0, 200 MM MGCL2 |
