4CFK

N-TERMINAL BROMODOMAIN OF HUMAN BRD4 WITH LY294002

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-11
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	37.450, 43.680, 78.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.250 - 1.550
R-factor	0.23221
Rwork	0.230
R-free	0.26736
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	REFMAC (5.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.250	1.600
High resolution limit [Å]	1.550	1.550
Rmerge	0.070	0.740
Number of reflections	19320
<I/σ(I)>	12.9	2
Completeness [%]	99.8	99.9
Redundancy	6.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			293	0.2M AMMONIUM SULPHATE, 15% PEG4K, 0.1M TRI-CITRATE PH 5.6 AT 20C