4CEI
Crystal structure of ADPNP-bound AddAB with a forked DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.944, 151.335, 124.560 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 29.949 - 2.800 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u44 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.749 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (PHASER-MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.930 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 68664 | |
| <I/σ(I)> | 15.9 | 3.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 0.1M HEPES PH 7.5, 17.5 % PEG 1500, 0.25M NACL, 0.1M SODIUM FORMATE, 0.1M MGCL2, VAPOR DIFFUSION, HANGING DROP, 285K |
