4CDK

Structure of ZNRF3-RSPO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1
Unit cell lengths	51.700, 80.160, 82.980
Unit cell angles	66.27, 81.36, 80.66

Refinement procedure

Resolution	44.706 - 2.800
R-factor	0.2192
Rwork	0.218
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.209
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.630	75.630
High resolution limit [Å]	2.800	2.800
Rmerge	0.090
Number of reflections	29157
Completeness [%]	95.4
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2M SODIUM BROMIDE AND 20% W/V PEG 3350