4CCZ
Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 166.803, 166.803, 166.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.350 - 2.700 |
| R-factor | 0.22004 |
| Rwork | 0.217 |
| R-free | 0.27408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q7q |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.320 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 1.200 |
| Number of reflections | 21347 | |
| <I/σ(I)> | 23.9 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4M SODIUM MALONATE |
