4CCH
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with d5SICS as templating nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.262, 114.262, 91.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.526 - 2.550 |
| R-factor | 0.2098 |
| Rwork | 0.207 |
| R-free | 0.26080 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3m8s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.770 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.700 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.200 | 1.440 |
| Number of reflections | 22906 | |
| <I/σ(I)> | 9.16 | 0.65 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 9 | 7.1 |
| CC(1/2) | 0.992 | 0.523 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20% W/V PEG 8000, 0.1M TRIS PH 8.0, 0.2M MAGNESIUM FORMATE, 20% GLYCEROL |
